5AVO

Crystal structure of the reduced form of homoserine dehydrogenase from Sulfolobus tokodaii.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2015-06-14
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	57.401, 79.472, 65.899
Unit cell angles	90.00, 107.16, 90.00

Refinement procedure

Resolution	50.000 - 1.800
R-factor	0.2129
Rwork	0.211
R-free	0.24700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ydr
RMSD bond length	0.008
RMSD bond angle	1.245
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	REFMAC
Refinement software	REFMAC (refmac_5.8.0049)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.830
High resolution limit [Å]	1.800	4.880	1.800
Rmerge	0.062	0.048	0.261
Rmeas	0.078	0.060	0.328
Rpim	0.047	0.035	0.196
Total number of observations	119944
Number of reflections	47593
<I/σ(I)>	13.7
Completeness [%]	90.6	75.4	94.8
Redundancy	2.5	2.5	2.4
CC(1/2)		0.993	0.911

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	PEG 2000, magnesium chloride, PEG 400, 1,4-butanediol