5AVH
The 0.90 angstrom structure (I222) of glucose isomerase crystallized in high-strength agarose hydrogel
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-07-28 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 0.70000 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 93.268, 97.588, 102.490 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 0.900 |
| R-factor | 0.11683 |
| Rwork | 0.116 |
| R-free | 0.12735 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.122 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 0.920 |
| High resolution limit [Å] | 0.900 | 0.900 |
| Rmerge | 0.073 | 0.354 |
| Number of reflections | 325304 | |
| <I/σ(I)> | 11.6 | 2.38 |
| Completeness [%] | 95.1 | 94.3 |
| Redundancy | 3 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7 | 293 | 0.1M Tris-HCl (pH 7.0), 10% (v/v) MPD, 0.1M Ca acetate |
