5AVC
human nucleosome core particle
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32XU |
Synchrotron site | SPring-8 |
Beamline | BL32XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-02-04 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 106.520, 110.120, 182.550 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.948 - 2.400 |
R-factor | 0.1995 |
Rwork | 0.198 |
R-free | 0.23790 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.656 |
Data reduction software | HKL-2000 |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.9_1690)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.115 | 1.295 |
Number of reflections | 84098 | |
<I/σ(I)> | 8.9 | 1.47 |
Completeness [%] | 99.7 | 99.83 |
Redundancy | 6.2 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6 | 293 | potassium cacodylate, manganese(II) chloride, potassium chloride |