5AV0

Crystal structure of DAPK1 in complex with 7,3',4'-trihydroxyisoflavone.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	46.732, 62.087, 88.101
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.900 - 1.850
R-factor	0.1885
Rwork	0.187
R-free	0.21980
RMSD bond length	0.005
RMSD bond angle	0.953
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	35.900
High resolution limit [Å]	1.850
Number of reflections	21813
<I/σ(I)>	23.7
Completeness [%]	96.6
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	2 M ammonium sulfate, 0.1 M MES