5ARN
Cu(I)-CSP3 (COPPER STORAGE PROTEIN 3) FROM METHYLOSINUS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-24 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 105.902, 105.902, 45.630 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 74.880 - 2.300 |
| R-factor | 0.18912 |
| Rwork | 0.186 |
| R-free | 0.24274 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5arm |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.494 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.910 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.080 | 0.650 |
| Number of reflections | 5998 | |
| <I/σ(I)> | 6.3 | 1.9 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 4.1 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 75 MM MGCL2, 100 MM HEPES PH 7.5, AND 34% PEG 400 |
