5ARM
APO-CSP3 (COPPER STORAGE PROTEIN 3) FROM METHYLOSINUS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-22 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 45.170, 105.500, 45.070 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.750 - 1.194 |
| R-factor | 0.138 |
| Rwork | 0.137 |
| R-free | 0.17100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kaw |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.885 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.270 | 1.210 |
| High resolution limit [Å] | 1.190 | 1.190 |
| Rmerge | 0.050 | 0.440 |
| Number of reflections | 34091 | |
| <I/σ(I)> | 8.5 | 2.2 |
| Completeness [%] | 98.7 | 99.1 |
| Redundancy | 3.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 40% PENTAERYTHRITOL PROPOXYLATE (5/4 PO/OH), 100 MM HEPES PH 7 AND 200 MM SODIUM THIOCYANATE |
