5AR3
RIP2 Kinase Catalytic Domain (1 - 310) complex with AMP-PCP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-09 |
| Detector | RIGAKU CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 131.566, 131.566, 106.582 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 3.230 |
| R-factor | 0.19218 |
| Rwork | 0.190 |
| R-free | 0.25426 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.224 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.290 |
| High resolution limit [Å] | 3.230 | 3.230 |
| Rmerge | 0.100 | 0.450 |
| Number of reflections | 72012 | |
| <I/σ(I)> | 14.3 | 3.2 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 4.1 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20% PEG3350, 0.2M NAKTARTRATE |
