5AQB
DARPin-based Crystallization Chaperones exploit Molecular Geometry as a Screening Dimension in Protein Crystallography
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-08-06 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 90.150, 96.170, 92.780 |
| Unit cell angles | 90.00, 118.99, 90.00 |
Refinement procedure
| Resolution | 48.085 - 1.370 |
| R-factor | 0.1574 |
| Rwork | 0.156 |
| R-free | 0.17680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 3DTM 1SVX CHAIN A AND 1GFL |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.985 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.080 | 1.420 |
| High resolution limit [Å] | 1.370 | 1.370 |
| Rmerge | 0.130 | |
| Number of reflections | 143759 | |
| <I/σ(I)> | 7 | 5.8 |
| Completeness [%] | 99.0 | 95 |
| Redundancy | 6.6 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | PEG3350 20.0% W/V, SODIUM FORMATE 0.2 M, BIS TRIS PROPANE 0.1 M, PH 8 |
