5APX
Sequence MATKDDIAN inserted between GCN4 adaptors - Structure T9(6)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-06 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 25.110, 38.250, 105.020 |
| Unit cell angles | 90.00, 93.33, 90.00 |
Refinement procedure
| Resolution | 34.950 - 1.800 |
| R-factor | 0.18893 |
| Rwork | 0.187 |
| R-free | 0.22597 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wpq |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.025 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.900 | 1.910 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.070 | 0.720 |
| Number of reflections | 18191 | |
| <I/σ(I)> | 14.5 | 2.14 |
| Completeness [%] | 97.1 | 95.4 |
| Redundancy | 3.94 | 3.81 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M AMMONIUM PHOSPHATE, 0.1 M TRIS PH 8.5, 50 %(V/V) MPD |
