5APQ
Sequence IENKAD inserted between GCN4 adaptors - Structure A6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-06 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 60.370, 34.810, 104.160 |
| Unit cell angles | 90.00, 101.09, 90.00 |
Refinement procedure
| Resolution | 30.040 - 2.100 |
| R-factor | 0.20996 |
| Rwork | 0.208 |
| R-free | 0.25143 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wpq |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.809 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.230 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.050 | 0.620 |
| Number of reflections | 12389 | |
| <I/σ(I)> | 15.5 | 2.28 |
| Completeness [%] | 97.3 | 96.2 |
| Redundancy | 3.71 | 3.71 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 95 MM TRI-SODIUM CITRATE PH 5.6, 19 %(V/V) ISOPROPANOL, 19 %(W/V) PEG 4000, 5 %(V/V) GLYCEROL |
