5AP3
Naturally Occurring Mutations in the MPS1 Gene Predispose Cells to Kinase Inhibitor Drug Resistance.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-05-23 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 70.730, 111.250, 112.010 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 59.690 - 2.700 |
| R-factor | 0.192 |
| Rwork | 0.190 |
| R-free | 0.23620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4c4j |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.070 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.690 | 2.830 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.090 | |
| Number of reflections | 12109 | |
| <I/σ(I)> | 9.3 | 1 |
| Completeness [%] | 97.4 | 99.4 |
| Redundancy | 3.9 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 38% (V/V) PEG300, pH 7.5 |
