5AOQ
Structural basis of neurohormone perception by the receptor tyrosine kinase Torso
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-08-01 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.420, 90.960, 244.280 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.975 - 2.700 |
| R-factor | 0.3428 |
| Rwork | 0.342 |
| R-free | 0.36220 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.748 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXD |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 200.000 | 2.780 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.110 | |
| Number of reflections | 33621 | |
| <I/σ(I)> | 14.7 | 0.4 |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 7.2 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 1.5-2.0 M AMMONIUM SULFATE, 100 MM 2-(N-MORPHOLINO)ETHANESULFONIC ACID [MES], PH 5.5 |
