5AO6
Endo180 D1-4, trigonal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-08-09 |
| Detector | ADSC CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 87.000, 87.000, 321.070 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 68.206 - 3.360 |
| R-factor | 0.2246 |
| Rwork | 0.220 |
| R-free | 0.30620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ao5 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.982 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.300 | 3.450 |
| High resolution limit [Å] | 3.360 | 3.360 |
| Rmerge | 0.180 | 0.970 |
| Number of reflections | 20831 | |
| <I/σ(I)> | 10.8 | 2.3 |
| Completeness [%] | 99.4 | 99.4 |
| Redundancy | 5.5 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | PH 7.5 |
