5ANN
Structure of fructofuranosidase from Xanthophyllomyces dendrorhous
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-14 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 74.620, 205.180, 145.770 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 145.770 - 2.140 |
| R-factor | 0.16995 |
| Rwork | 0.169 |
| R-free | 0.18790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ldk |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.372 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.920 | 2.260 |
| High resolution limit [Å] | 2.140 | 2.140 |
| Rmerge | 0.110 | 0.390 |
| Number of reflections | 117631 | |
| <I/σ(I)> | 12.8 | 4.3 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 7.1 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | PROTEIN WAS CRYSTALLIZED FROM 1.3M SODIUM CITRATE TRIBASIC DIHYDRATE, 0.1M HEPES PH 7.5 |
