5ANG

Crystal structure of CDK2 in complex with 7-hydroxy-4-(morpholinomethyl)chromen-2-one processed with the CrystalDirect automated mounting and cryo-cooling technology

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 21 21 21
Unit cell lengths	53.711, 71.828, 72.351
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.900
R-factor	0.19862
Rwork	0.197
R-free	0.23569
Structure solution method	OTHER
Starting model (for MR)	NONE
RMSD bond length	0.019
RMSD bond angle	2.066
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall
Low resolution limit [Å]	29.850
High resolution limit [Å]	1.900
Rmerge	0.070
Number of reflections	22707
Completeness [%]	99.9
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				0.02 M HEPES, PH=7.0, 5% GLYCEROL, 12% PEG 3350