5AN3
Structure of an Sgt1-Skp1 Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC ADSC Q105 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 94.560, 94.560, 122.990 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.880 - 2.820 |
| R-factor | 0.2043 |
| Rwork | 0.202 |
| R-free | 0.24070 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.980 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | Auto-Rickshaw |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.890 | 2.890 |
| High resolution limit [Å] | 2.820 | 2.820 |
| Rmerge | 0.090 | 0.830 |
| Number of reflections | 15826 | |
| <I/σ(I)> | 29.2 | 4.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 21.5 | 21.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.325 M MGCL2, 22.5% PEG-6000, 0.1 M TRIS-HCL PH 8 |
