5AMX

Crystal Structure of Proteinase K processed with the CrystalDirect automated mounting and cryo-cooling technology

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315r
Spacegroup name	P 43 21 2
Unit cell lengths	67.975, 67.975, 102.057
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.130 - 1.010
R-factor	0.15751
Rwork	0.157
R-free	0.16565
Structure solution method	OTHER
Starting model (for MR)	NONE
RMSD bond length	0.026
RMSD bond angle	1.973
Refinement software	REFMAC (5.8.0123)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.010
Rmerge	0.050
Number of reflections	113538
Completeness [%]	89.6
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				0.1 M HEPES, PH=7.5, 1.3 M AMMONIUM SULFATE, 0.1 M NACL