5AMW
Crystal Structure of the Strawberry Pathogenesis-Related 10 (PR-10) Fra a 2 protein (A141F) processed with the CrystalDirect automated mounting and cryo-cooling technology
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF |
| Synchrotron site | ESRF |
| Temperature [K] | 100 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 105.559, 41.463, 92.058 |
| Unit cell angles | 90.00, 116.28, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.900 |
| R-factor | 0.22319 |
| Rwork | 0.221 |
| R-free | 0.26493 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.838 |
| Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 1.900 |
| Rmerge | 0.080 |
| Number of reflections | 28520 |
| Completeness [%] | 99.9 |
| Redundancy | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M BIS-TRIS,PH=5.5, 0.2 M AMMONIUM SULFATE, 25% PEG 3350, |
