5AMQ
Structure of the La Crosse Bunyavirus polymerase in complex with the 3' and 5' viral RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2014-09-04 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 103.000, 141.100, 165.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.170 - 3.000 |
| R-factor | 0.19516 |
| Rwork | 0.192 |
| R-free | 0.25242 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.148 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.120 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.010 | 0.080 |
| Number of reflections | 48549 | |
| <I/σ(I)> | 11.91 | 1.55 |
| Completeness [%] | 99.5 | 99.5 |
| Redundancy | 4.32 | 4.52 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.1M HEPES PH 7, 0.2M SODIUM CITRATE DIHYDRATE, 0.3M AMMONIUM SULPHATE, 15% PEG 3350 |
