5AMP
Geotrichum candidum Cel7A apo structure at 2.1A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-09-26 |
| Detector | CHESS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.880, 90.560, 55.270 |
| Unit cell angles | 90.00, 109.86, 90.00 |
Refinement procedure
| Resolution | 51.980 - 2.120 |
| R-factor | 0.14863 |
| Rwork | 0.146 |
| R-free | 0.19415 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gpi |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.077 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.980 | 2.140 |
| High resolution limit [Å] | 2.030 | 2.110 |
| Rmerge | 0.060 | 0.550 |
| Number of reflections | 24567 | |
| <I/σ(I)> | 13.9 | |
| Completeness [%] | 95.5 | 75.1 |
| Redundancy | 3.7 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.9 | 0.2M MAGNESIUM CHLORIDE HEXAHYDRATE, 20% PEG 3350, pH 5.9 |
