5AMK
Cereblon isoform 4 from Magnetospirillum gryphiswaldense in multiple conformations, hexagonal crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-06-24 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 137.860, 137.860, 154.390 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.980 - 2.900 |
| R-factor | 0.17664 |
| Rwork | 0.175 |
| R-free | 0.21688 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4v2y |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.952 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.000 | 3.070 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.100 | 0.530 |
| Number of reflections | 19746 | |
| <I/σ(I)> | 10.53 | 2.22 |
| Completeness [%] | 99.1 | 98.3 |
| Redundancy | 2.97 | 2.95 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 64 MM SODIUM CITRATE PH 7.0, 100 MM HEPES PH 7.0, 10 %(W/V) PEG 5000 MME |
