5AMJ
Cereblon isoform 4 from Magnetospirillum gryphiswaldense in complex with Thalidomide, Wash II structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-12 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.350, 59.430, 86.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.240 - 1.750 |
| R-factor | 0.1567 |
| Rwork | 0.155 |
| R-free | 0.19053 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4v2y |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.397 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.200 | 1.860 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.070 | 0.480 |
| Number of reflections | 30163 | |
| <I/σ(I)> | 9.79 | 2.16 |
| Completeness [%] | 97.7 | 96.5 |
| Redundancy | 2.86 | 2.79 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 100 MM TRI-SODIUM CITRATE PH 5.6, 1.0 M AMMONIUM PHOSPHATE |
