5AMI
Cereblon isoform 4 from Magnetospirillum gryphiswaldense in complex with Thalidomide, Wash I structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-13 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.250, 59.656, 86.728 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.290 - 1.750 |
| R-factor | 0.16727 |
| Rwork | 0.165 |
| R-free | 0.20942 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4v2y |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.235 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.300 | 1.860 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.050 | 0.680 |
| Number of reflections | 30628 | |
| <I/σ(I)> | 16.98 | 1.9 |
| Completeness [%] | 99.7 | 98.7 |
| Redundancy | 4.23 | 4.23 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 100 MM SODIUM ACETATE PH 4.6, 20 %(W/V) PEG 6000 |
