5AJS
Crystal structure of a coiled-coil domain from human THAP11
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-21 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 43 |
| Unit cell lengths | 39.757, 39.757, 204.127 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.760 - 2.300 |
| R-factor | 0.21236 |
| Rwork | 0.207 |
| R-free | 0.29516 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1n6m |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.708 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.760 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.040 | 0.440 |
| Number of reflections | 13992 | |
| <I/σ(I)> | 16.2 | 2.6 |
| Completeness [%] | 99.4 | 97.8 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 285 | 0.1 M NA/K PHOSPHATE PH 6.2, 0.2 M NACL, 40% (V/V) MPD, HANGING DROP VAPOUR DIFFUSION, T = 285 K |
