5AJL
Sdsa sulfatase tetragonal
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 43 |
| Unit cell lengths | 88.580, 88.580, 193.930 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.610 - 3.450 |
| R-factor | 0.24638 |
| Rwork | 0.244 |
| R-free | 0.28901 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cg3 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.252 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.680 |
| High resolution limit [Å] | 3.480 | 3.480 |
| Rmerge | 0.100 | 0.830 |
| Number of reflections | 18672 | |
| <I/σ(I)> | 12.11 | 1.65 |
| Completeness [%] | 97.6 | 88.9 |
| Redundancy | 5 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 12% PEG 4000, 10% ISOPROPANOL, 200 MM LICL, 100 MM CITRATE BUFFER PH 6.0. |
