5AJH
Crystal structure of Fusarium oxysporum cutinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-07-17 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.906, 60.141, 241.965 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 120.980 - 1.900 |
| R-factor | 0.18355 |
| Rwork | 0.181 |
| R-free | 0.22373 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cex |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.212 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 120.900 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.230 | 1.270 |
| Number of reflections | 42540 | |
| <I/σ(I)> | 7.5 | 1.5 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 8.6 | 8.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.12M MONOSACCHARIDES, PH 8.5 12.5% MPD, 12.5% PEG1000, 12.5% PEG3350 |
