5AJG
Structure of Infrared Fluorescent Protein IFP1.4 AT 1.11 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-11-21 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 95.361, 53.028, 66.219 |
| Unit cell angles | 90.00, 90.90, 90.00 |
Refinement procedure
| Resolution | 38.430 - 1.110 |
| R-factor | 0.14693 |
| Rwork | 0.146 |
| R-free | 0.16806 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4cqh |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.944 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.400 | 1.130 |
| High resolution limit [Å] | 1.110 | 1.110 |
| Number of reflections | 121711 | |
| <I/σ(I)> | 17.6 | 2 |
| Completeness [%] | 98.5 | 92.1 |
| Redundancy | 4.3 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 26% PEG 400, 0.1 M SODIUM ACETATE PH 5.0 |
