5AJC
X-ray structure of RSL lectin in complex with sialyl lewis X tetrasaccharide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-26 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.766, 83.163, 90.697 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.070 - 1.700 |
| R-factor | 0.15257 |
| Rwork | 0.151 |
| R-free | 0.18673 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bt9 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.716 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.530 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.050 | 0.340 |
| Number of reflections | 31053 | |
| <I/σ(I)> | 14.5 | 3.1 |
| Completeness [%] | 99.1 | 99.9 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 30% PEG 6K 280MM TRIS PH 8.5 |
