5AJ8

Tubulin Binding Cofactor C from Leishmania major

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-09-22
Detector	DECTRIS PILATUS
Spacegroup name	P 1 21 1
Unit cell lengths	37.644, 93.236, 48.277
Unit cell angles	90.00, 108.38, 90.00

Refinement procedure

Resolution	46.620 - 2.200
R-factor	0.15758
Rwork	0.155
R-free	0.19738
Structure solution method	SAD
Starting model (for MR)	NONE
RMSD bond length	0.010
RMSD bond angle	1.326
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX (AUTOSOL)
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.620	2.270
High resolution limit [Å]	2.200	2.200
Rmerge	0.100	0.240
Number of reflections	15839
<I/σ(I)>	17.5	6.8
Completeness [%]	98.3	86.8
Redundancy	11.9	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1