5AIR

Structural analysis of mouse GSK3beta fused with LRP6 peptide.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-23
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	82.902, 85.883, 177.471
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.530
R-factor	0.21451
Rwork	0.212
R-free	0.25983
Structure solution method	OTHER
RMSD bond length	0.014
RMSD bond angle	1.726
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall
Low resolution limit [Å]	177.500
High resolution limit [Å]	2.530
Rmerge	0.140
Number of reflections	42941
<I/σ(I)>	15.37
Completeness [%]	98.0
Redundancy	9.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	290	200 MM SODIUM MALONATE, 20 % (V/V) PEG 3350, pH 7