5AIM
Crystal structure of T138 central eWH domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 129.090, 129.090, 68.040 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.191 - 1.401 |
| R-factor | 0.1763 |
| Rwork | 0.175 |
| R-free | 0.19750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | STRUCTURE OF THIS PROTEIN BY SULPHUR-SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.947 |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.430 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.050 | 1.380 |
| Number of reflections | 42553 | |
| <I/σ(I)> | 1.7 | |
| Completeness [%] | 99.9 | 99.3 |
| Redundancy | 10.1 | 9.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 1.15 M NA CITRATE PH 6.2, 0.1 M NA CACODYLATE |
