5AIF
Discovery and characterization of thermophilic limonene-1,2-epoxide hydrolases from hot spring metagenomic libraries. Tomsk-sample-Native
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | P 65 |
| Unit cell lengths | 96.370, 96.370, 57.810 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.730 - 1.260 |
| R-factor | 0.11846 |
| Rwork | 0.117 |
| R-free | 0.13797 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nww |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.301 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.700 | 1.290 |
| High resolution limit [Å] | 1.260 | 1.260 |
| Rmerge | 0.060 | 1.040 |
| Number of reflections | 82450 | |
| <I/σ(I)> | 20.4 | 2.1 |
| Completeness [%] | 99.9 | 99.1 |
| Redundancy | 10.1 | 9.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
