5AID
Crystal structure of the Mep2 mutant delta442 from Candida albicans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-04 |
| Detector | DEXTRIS |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 114.733, 114.733, 289.964 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 96.655 - 3.400 |
| R-factor | 0.2409 |
| Rwork | 0.236 |
| R-free | 0.28920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5aex |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.283 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 96.650 | 3.670 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.100 | 1.170 |
| Number of reflections | 10453 | |
| <I/σ(I)> | 14.6 | 2.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.3 | 9.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 24% PEG400, 0.05 M SODIUM ACETATE, 0.05 M MAGNESIUM ACETATE PH 6.1 |
