5AHY
Halorhodopsin from Halobacterium salinarum in a new rhombohedral crystal form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-07-24 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | H 3 2 |
Unit cell lengths | 103.651, 103.651, 138.588 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.699 - 2.150 |
R-factor | 0.1873 |
Rwork | 0.185 |
R-free | 0.22720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1e12 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.071 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.700 | 2.230 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.090 | 0.760 |
Number of reflections | 15809 | |
<I/σ(I)> | 14.9 | 2.1 |
Completeness [%] | 99.9 | 99.7 |
Redundancy | 4.9 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 296 | RESERVOIR SOLUTION: 100 MM CITRATE PH 8, 150 MM NACL, 2.1-2.4 M (NH4)2SO4. VAPOR DIFFUSION AT 296 K WITH DROP RATIO OF 1.2 TO 0.8 UL PROTEIN TO RESERVOIR. PROTEIN CONCENTRATION 6 MG/ML. |