5AHW
Crystal structure of universal stress protein MSMEG_3811 in complex with cAMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-23 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 82.530, 126.780, 151.720 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.640 - 2.150 |
| R-factor | 0.18158 |
| Rwork | 0.179 |
| R-free | 0.23520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2z08 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.901 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.640 | 2.210 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.080 | 0.900 |
| Number of reflections | 43084 | |
| <I/σ(I)> | 15.1 | 1.8 |
| Completeness [%] | 98.8 | 98.5 |
| Redundancy | 4.5 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | PROTEIN WAS COCRYSTALLIZED WITH 5 MM CAMP IN 0.1 M MES, PH 6.0; 1.9 M AMMONIUM SULFATE; 6 % (V/V) POLYPROPYLENGLYCOL 400. |
