5AGX

Bcl-2 alpha beta-1 LINEAR complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-09-19
Detector	ADSC CCD
Spacegroup name	P 43 21 2
Unit cell lengths	86.054, 86.054, 108.739
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.009 - 2.240
R-factor	0.1906
Rwork	0.188
R-free	0.24470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	UNPUBLISHED BCL-2 BECLIN BH3 COMPLEX
RMSD bond length	0.007
RMSD bond angle	1.105
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.320
High resolution limit [Å]	2.240	2.240
Rmerge	0.070	0.780
Number of reflections	20251
<I/σ(I)>	29.2	3.89
Completeness [%]	99.9	99.2
Redundancy	14.2	14

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7		PH 7.0