5AGC
Crystallographic forms of the Vps75 tetramer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-09 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 82.750, 92.430, 160.750 |
| Unit cell angles | 90.00, 93.85, 90.00 |
Refinement procedure
| Resolution | 50.060 - 4.000 |
| R-factor | 0.23104 |
| Rwork | 0.229 |
| R-free | 0.26463 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zd7 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.397 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.060 | 4.220 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Rmerge | 0.140 | 0.480 |
| Number of reflections | 9771 | |
| <I/σ(I)> | 7 | 3.4 |
| Completeness [%] | 99.0 | 96.4 |
| Redundancy | 5.1 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1M SODIUM CACODYLATE PH 6.5, 25% W/V PEG 4K |
