5AG9
CRYSTAL STRUCTURE OF A MUTANT (665sXa) C-TERMINAL DOMAIN OF RGPB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-15 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.570, 61.810, 54.050 |
| Unit cell angles | 90.00, 116.79, 90.00 |
Refinement procedure
| Resolution | 48.250 - 2.110 |
| R-factor | 0.19538 |
| Rwork | 0.192 |
| R-free | 0.25410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ag8 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.924 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.030 | 2.220 |
| High resolution limit [Å] | 2.110 | 2.110 |
| Rmerge | 0.150 | 0.450 |
| Number of reflections | 17553 | |
| <I/σ(I)> | 6.4 | 2.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.8 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 30% PEG 4000,0.2 M (NH4)2SO4 |
