5AG0
DyP-type peroxidase of Auricularia auricula-judae (AauDyPI) crystallized at pH 6.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-28 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.840, 46.570, 147.630 |
| Unit cell angles | 90.00, 100.49, 90.00 |
Refinement procedure
| Resolution | 47.510 - 1.750 |
| R-factor | 0.18913 |
| Rwork | 0.187 |
| R-free | 0.22225 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4au9 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.672 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.510 | 1.860 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.080 | 0.460 |
| Number of reflections | 86374 | |
| <I/σ(I)> | 12.31 | 2.31 |
| Completeness [%] | 96.4 | 90.5 |
| Redundancy | 2.4 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.17 M SODIUM ACETATE TRIHYDRATE, 0.085 M SODIUM CACODYLATE PH 6.5, 25.5% (W/V) PEG 8000, 15% (V/V) GLYCEROL |
