5AEO
Virulence-associated protein VapG from the intracellular pathogen Rhodococcus equi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 120 |
| Detector technology | PIXEL |
| Collection date | 2013-03-17 |
| Detector | DECTRIS PIXEL |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 44.550, 44.550, 259.670 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.500 - 1.800 |
| R-factor | 0.20094 |
| Rwork | 0.199 |
| R-free | 0.23623 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4csb |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.893 |
| Data reduction software | MOSFLM |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.500 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.040 | 0.670 |
| Number of reflections | 24979 | |
| <I/σ(I)> | 31 | 2.6 |
| Completeness [%] | 98.0 | 83 |
| Redundancy | 11.4 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | PROTEIN SOLUTION: 30 MG/ML VAPG IN 10 MM TRIS-HCL PH 7.5, 10 MM NACL RESERVOIR: 0.3 M KCL, 20 % W/V PEG 3350 |
