5AEI
Designed Armadillo repeat protein YIIIM5AII in complex with peptide (KR)5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-05-14 |
| Detector | DECTRIS PILATUS 2M-F |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 88.830, 215.280, 105.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 107.640 - 1.830 |
| R-factor | 0.15015 |
| Rwork | 0.149 |
| R-free | 0.17914 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4plr |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.052 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.880 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 0.180 | 1.500 |
| Number of reflections | 89570 | |
| <I/σ(I)> | 14.24 | 2.02 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.1 | 11.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.2 M CA-ACETATE, 0.1 M TRIS (HOAC), 8% PEG 20K, 8% PEG 550MME, PH 7.5 |
