5AEG
A bacterial protein structure in glycoside hydrolase family 31.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2015-04-28 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.665, 112.643, 111.881 |
| Unit cell angles | 90.00, 109.52, 90.00 |
Refinement procedure
| Resolution | 105.450 - 1.850 |
| R-factor | 0.16189 |
| Rwork | 0.160 |
| R-free | 0.19337 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5aed |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.858 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0095) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.680 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.050 | 0.620 |
| Number of reflections | 154198 | |
| <I/σ(I)> | 13.5 | 2.1 |
| Completeness [%] | 98.6 | 95.7 |
| Redundancy | 4 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 293 | 25 MG ML-1 YIHQ PROTEIN STOCK IS MIXED WITH EQUAL VOLUME OF PRECIPITANT COMPOSED OF 50-60% (V/V) 2-METHYL-2,4-PENTANEDIOL, 0.1-0.15 M CACL2, AND BIS-TRIS, (PH 6.5) AFTER 3-4 DAYS AT 20 DEGREE. THE LIGAND I5F WAS SOAKED INTO THE NATIVE CRYSTAL. |
