5AED
A bacterial protein structure in glycoside hydrolase family 31
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2014-11-16 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.710, 113.177, 111.720 |
| Unit cell angles | 90.00, 109.23, 90.00 |
Refinement procedure
| Resolution | 105.490 - 1.910 |
| R-factor | 0.1697 |
| Rwork | 0.168 |
| R-free | 0.20134 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.813 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.060 | 1.940 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rmerge | 0.080 | 0.540 |
| Number of reflections | 138269 | |
| <I/σ(I)> | 7.9 | 2 |
| Completeness [%] | 96.8 | 99.2 |
| Redundancy | 2.8 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 293 | 25 MG/ML PROTEIN IN THE BUFFER OF 50 MM NAPO4 BUFFER PH 7.5 AND 250 MM NACL WAS MIXED 1:1 WITH THE PRECIPITANT COMPOSED OF 50-60% MPD, 0.1-0.15M CACL2 AND BIS-TRIS PH 6.5. TEMP: 20 C |
