5AE5
Structures of inactive and activated DntR provide conclusive evidence for the mechanism of action of LysR transcription factors
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 107.132, 107.132, 297.844 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.142 - 2.645 |
| R-factor | 0.2009 |
| Rwork | 0.199 |
| R-free | 0.22720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1utb |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.969 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.140 | 2.790 |
| High resolution limit [Å] | 2.640 | 2.640 |
| Rmerge | 0.100 | 1.000 |
| Number of reflections | 30522 | |
| <I/σ(I)> | 13.5 | 2.1 |
| Completeness [%] | 97.7 | 98.1 |
| Redundancy | 7.9 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 0.2 M SODIUM POTASSIUM TARTRATE, 0.1 M TRIS-HCL PH 8.0, 5 % (W/V) PEG 6000 |
