5ADV
The Periplasmic Binding Protein CeuE of Campylobacter jejuni preferentially binds the iron(III) complex of the Linear Dimer Component of Enterobactin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-11-30 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 |
| Unit cell lengths | 58.070, 63.090, 67.160 |
| Unit cell angles | 83.09, 76.90, 79.21 |
Refinement procedure
| Resolution | 65.190 - 2.100 |
| R-factor | 0.22039 |
| Rwork | 0.218 |
| R-free | 0.26021 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zkw |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.656 |
| Data reduction software | xia2 |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.590 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.090 | 0.810 |
| Number of reflections | 51094 | |
| <I/σ(I)> | 7.7 | 1.7 |
| Completeness [%] | 96.3 | 95.4 |
| Redundancy | 2.1 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | 0.1 M PCB (SODIUM PROPIONATE, SODIUM CACODYLATE, BIS-TRIS PROPANE, 2:1:2) BUFFER PH 9.0 25% (W/V) PEG 1500 |
