5ACZ

COMPLEX OF A B21 CHICKEN MHC CLASS I MOLECULE AND A 11MER CHICKEN PEPTIDE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	CCD
Collection date	2011-01-17
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	71.610, 72.328, 72.815
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.310 - 2.690
R-factor	0.24007
Rwork	0.239
R-free	0.26697
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3bev
RMSD bond length	0.007
RMSD bond angle	1.125
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0124)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	72.330	2.820
High resolution limit [Å]	2.690	2.690
Rmerge	0.150	0.690
Number of reflections	10877
<I/σ(I)>	12	2.7
Completeness [%]	99.4	97.7
Redundancy	5.3	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5		MOLECULAR DIMENSIONS E9, PH 8.5