5ACS
Y233A-Investigation of the impact from residues W228 and Y233 in the metallo-beta-lactamase GIM-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 38.434, 131.222, 40.842 |
Unit cell angles | 90.00, 94.83, 90.00 |
Refinement procedure
Resolution | 24.914 - 1.459 |
R-factor | 0.1292 |
Rwork | 0.128 |
R-free | 0.16710 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ynt |
RMSD bond length | 0.007 |
RMSD bond angle | 0.995 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.000 | 1.540 |
High resolution limit [Å] | 1.460 | 1.460 |
Rmerge | 0.040 | 0.460 |
Number of reflections | 68700 | |
<I/σ(I)> | 12.2 | 2.2 |
Completeness [%] | 98.4 | 95.8 |
Redundancy | 3.8 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 28% PEG 4000, 0.1 M HEPES PH 7.0 AND POTASSIUM CHLORIDE |