5ACI
X-ray Structure of LPMO
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 41 3 2 |
| Unit cell lengths | 125.460, 125.460, 125.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.750 |
| R-factor | 0.16634 |
| Rwork | 0.165 |
| R-free | 0.19545 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4eir |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.667 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.800 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.210 | 1.850 |
| Number of reflections | 34560 | |
| <I/σ(I)> | 21.72 | 2.59 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 35.5 | 31.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 3.6M SODIUM CHLORIDE, 100MM CITRIC ACID PH4.0, pH 5.5 |
