5ACH
X-ray Structure of LPMO
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | P 41 3 2 |
| Unit cell lengths | 124.700, 124.700, 124.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.570 - 1.280 |
| R-factor | 0.11903 |
| Rwork | 0.118 |
| R-free | 0.14705 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4eir |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.971 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.570 | 1.320 |
| High resolution limit [Å] | 1.280 | 1.280 |
| Rmerge | 0.080 | 1.080 |
| Number of reflections | 85084 | |
| <I/σ(I)> | 13.9 | 1.4 |
| Completeness [%] | 99.8 | 99.3 |
| Redundancy | 7.3 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 3.0M SODIUM CHLORIDE, 100MM CITRIC ACID PH3.5, pH 5.5 |
