5AC8
S. enterica HisA with mutations D10G, dup13-15, G102A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-25 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 86.415, 86.415, 122.027 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.289 - 1.699 |
| R-factor | 0.1969 |
| Rwork | 0.196 |
| R-free | 0.20920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ahf |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.152 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.140 | 1.390 |
| Number of reflections | 30311 | |
| <I/σ(I)> | 14.96 | 1.67 |
| Completeness [%] | 99.8 | 99 |
| Redundancy | 19 | 17.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 2.0M AMMONIUM SULFATE, 0.1M SODIUM ACETATE PH4.6 |
